Review



m-mlv reverse transcriptase (rnase h(-), point mutant enzyme  (Promega)

 
  • Logo
  • About
  • News
  • Press Release
  • Team
  • Advisors
  • Partners
  • Contact
  • Bioz Stars
  • Bioz vStars
  • 90

    Structured Review

    Promega m-mlv reverse transcriptase (rnase h(-), point mutant enzyme
    M Mlv Reverse Transcriptase (Rnase H( ), Point Mutant Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-mlv+transcriptase+enzyme(h)/m+mlv+reverse+transcriptase/10__3233_slash_jbr___160129-44-48-60
    Average 90 stars, based on 1 article reviews
    m-mlv reverse transcriptase (rnase h(-), point mutant enzyme - by Bioz Stars, 2026-09
    90/100 stars

    Images

    Related Articles

    Polymerase Chain Reaction:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Selection:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Construct:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Recombinant:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Reverse Transcription:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Software:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Virus:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Mutagenesis:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Incubation:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Real-time Polymerase Chain Reaction:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    SYBR Green Assay:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).

    Sterility:

    Article Title: Sensizitation of cancer cells to therapy using sina targeting genes from the 1P and 19Q chromosomal regions
    Article Snippet: RNAs were reverse transcribed using the M-MLV reverse transcriptase RNase H minus enzyme (Promega) and oligodT as a primer, at 40 deg.

    Article Title: Diversity Scale of Library Matters: Impact of mRNA Library Diversity Scales on the Discovery of Macrocyclic Peptides Targeting a Protein by the RaPID System
    Article Snippet: Reagent Source Identifier Oligonucleotides PCR primers used for the library construction or the RaPID system selection, see Table S2 Eurofins Genomics, Tokyo, Japan N/A Puromycin construct: 5’- pCTCCCGCCCCCCGTCC–PEG linker– CC–puromycin-3’ Gene Design Inc, Osaka, Japan N/A Recombinant Proteins and enzymes His-/ hFc-tagged human MET ectodomain Sino Biological Inc. 10692-H03H Recombinant human IgG1 Fc R&D systems 110-HG-100 M-MLV reverse transcriptase (H-) Promega M3682 RNasin RNase inhibitor Promega N2615 RQ1 DNase Promega M6101 Enzymes without further specification Expressed by Suga Group members N/A Critical Commercial Assays Series S Sensor Chip Protein G Cytiva 29179315 MiSeq Reagent Kit v3 (150-cycle) illumina MS-102-3001 TapeStation D1000 ScreenTape/ TapeStation D1000 Reagent Kit Agilent 5067-5582/ 5067-5583 Chemicals Chemicals for premixed buffers and other procedures Nacalai Tesque, Tokyo Chemical Industry, Sigma-Aldrich Japan, or Fujifilm Wako Chemicals N/A Amino acid derivatives and coupling reagents for SPPS Watanabe Chemical Co., Ltd N/A Software and Online Tools BiacoreTM Insight Software Cytiva 29310602 WEBLOGO 2.8.2 https://weblogo.berkeley.edu/ N/A

    Article Title: Role of mechanical stretching and lipopolysaccharide in early apoptosis and IL-8 of alveolar epithelial type Π cells A549
    Article Snippet: One microgram of total RNA was heated at 70 曟 for 5 min. and placed on ice for 5 min. A mix of 5 毺L M-MLV RT 5暳 reaction buffer, 10 mM of each of the 4 dNTPs, 200 U of M-MLV transcriptase enzyme(H-), 25 pg/毺L of oligo (dT) primer and 20 U of RNase inhibitor (all from Promega, WI, USA) was added to each sample, followed by incubation at 40 曟 for 60 min and 70 曟 for 15 min. Real-time PCR was performed using double stranded DNA dye SYBR Green PCR Master Mix (PE Biosystems, Warrington, UK) on the ABI PRISM 7700 system (PerkinElmer, CA,USA).

    Article Title: Gambogic acid and gambogenic acid induce a thiol-dependent heat shock response and disrupt the interaction between HSP90 and HSF1 or HSF2
    Article Snippet: The reverse transcriptase enzyme M-MLV RT (H-) (Moloney Murine Leukemia Virus Reverse Transcriptase RNase H minus, Promega) was then used to transcribe 1 μg of total RNA to cDNA using Oligo(dT) 15 primers (Promega).

    Article Title: Variation in single-nucleotide sensitivity of eCLIP derived from reverse transcription conditions
    Article Snippet: To each was added 4 μL H 2 O, 4 μL 5X M-MLV buffer (Promega), 1 μL 10 mM dNTPs, 0.2 μL Murine RNase Inhibitor (NEB), and 0.8 μL of M-MLV enzyme (Promega M-MLV Reverse Transcriptase, RNase H Minus, Point Mutant).

    Article Title: Norovirus genotypes in endemic acute gastroenteritis of infants and children in Finland between 1994 and 2007
    Article Snippet: The RT reaction was performed at 42°C for 60 min with 2·5 μl RNA added to the RT mixture (total volume 50 μl) containing 22·9 μl sterile water, 1×GeneAmp PCR buffer (Applied Biosystems, USA), 1·5 m m GeneAmp MgCl 2 (Applied Biosystems), 400 μ m dNTPs each, 16 ng/μl p289H,I,IUB reverse primer mixture, 10 U RNasin® (Promega, USA) and 70 U M-MLV Reverse Transcriptase RNase H − enzyme (Promega).

    Article Title: Lactobacillus salivarius and L. gasseri down-regulate Aggregatibacter actinomycetemcomitans exotoxins expression
    Article Snippet: This was then reversed transcribed into cDNA, by using Moloney murine leukemia virus reverse-transcriptase enzyme (M-MLV RT RNase H minus) (Promega, Madison, Wi, USA).



    Similar Products

    90
    Promega m-mlv reverse transcriptase (rnase h(-), point mutant enzyme
    M Mlv Reverse Transcriptase (Rnase H( ), Point Mutant Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-mlv+transcriptase+enzyme(h)/m+mlv+reverse+transcriptase/10__3233_slash_jbr___160129-44-48-60
    Average 90 stars, based on 1 article reviews
    m-mlv reverse transcriptase (rnase h(-), point mutant enzyme - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Promega moloney murine leukemia virus reverse-transcriptase enzyme m-mlv rt rnase h minus
    Moloney Murine Leukemia Virus Reverse Transcriptase Enzyme M Mlv Rt Rnase H Minus, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-mlv+transcriptase+enzyme(h)/m+mlv+reverse+transcriptase/pmc04028514-40-9-20
    Average 90 stars, based on 1 article reviews
    moloney murine leukemia virus reverse-transcriptase enzyme m-mlv rt rnase h minus - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Promega m-mlv reverse transcriptase rnase h minus enzyme
    M Mlv Reverse Transcriptase Rnase H Minus Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-mlv+transcriptase+enzyme(h)/m+mlv+reverse+transcriptase/us08293886-109-6-13
    Average 90 stars, based on 1 article reviews
    m-mlv reverse transcriptase rnase h minus enzyme - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Promega m-mlv transcriptase enzyme(h)
    M Mlv Transcriptase Enzyme(h), supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-mlv+transcriptase+enzyme(h)/m+mlv+reverse+transcriptase/10__1016_slash_s1995___7645_ascii40_12_ascii41_60131___x-55-41-58
    Average 90 stars, based on 1 article reviews
    m-mlv transcriptase enzyme(h) - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Promega m-mlv reverse transcriptase rnase h− enzyme
    M Mlv Reverse Transcriptase Rnase H− Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-mlv+transcriptase+enzyme(h)/m+mlv+reverse+transcriptase/pmc03243036-43-61-68
    Average 90 stars, based on 1 article reviews
    m-mlv reverse transcriptase rnase h− enzyme - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Promega m-mlv rnase-h − point mutant reverse transcriptase enzyme
    M Mlv Rnase H − Point Mutant Reverse Transcriptase Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-mlv+transcriptase+enzyme(h)/m+mlv+reverse+transcriptase/pmc03023762-118-25-33
    Average 90 stars, based on 1 article reviews
    m-mlv rnase-h − point mutant reverse transcriptase enzyme - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Promega m-mlv rnase h-point mutant reverse transcriptase enzyme
    M Mlv Rnase H Point Mutant Reverse Transcriptase Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-mlv+transcriptase+enzyme(h)/m+mlv+reverse+transcriptase/pm18367189-82-29-36
    Average 90 stars, based on 1 article reviews
    m-mlv rnase h-point mutant reverse transcriptase enzyme - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    90
    Promega m-mlv reverse transcriptase rnase h minus-enzyme
    M Mlv Reverse Transcriptase Rnase H Minus Enzyme, supplied by Promega, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/m-mlv+transcriptase+enzyme(h)/m+mlv+reverse+transcriptase/pmc02801008-104-9-38
    Average 90 stars, based on 1 article reviews
    m-mlv reverse transcriptase rnase h minus-enzyme - by Bioz Stars, 2026-09
    90/100 stars
      Buy from Supplier

    Image Search Results